The model plant thale cress acquires organic nitrogen from the soil, although its roots do not form symbiosis with mycorrhizal fungi. This is shown in a study, led by SLU researchers. They also show that plants can acquire organic nitrogen in competition with microorganisms in soil, which previously has been questioned. This means that organic nitrogen can contribute to the plant’s nitrogen supply, and raises the question if agricultural crops could be modified to become more efficient in using organic nitrogen as a source of nutrients. The study is published in a recent issue of the scientific journal Plant, Cell & Environment.
The classic science on plant nutrition states that plants acquire nitrogen from the soil as nitrate or ammonium, or as nitrogen gas if the plant forms symbiosis with nitrogen-fixing bacteria. Today we know that there are plants that also can make use of organic nitrogen, but the consensus has been that it only applies to certain mycorrhiza-forming plants growing in nutrient-poor soils. Together with colleagues from Austria and Australia the SLU researchers show that a plant that does not form mycorrhiza acquires organic nitrogen when grown in nutritious agricultural soil.
The new findings show that the plant is dependent on a specific protein for this to work. The protein is an amino acid transporter, and the researchers have performed a number of experiments on genetically modified thale cress (Arabidopsis thaliana) that either lack the transporter or over produces it. Torgny Näsholm, Professor in ecophysiology at the Swedish University of agricultural sciences in Umeå, led the study.
"We grew the plants in greenhouses in agricultural soil, and could follow the way of the amino acid glutamine from the soil into the plant by labelling the glutamine with carbon and nitrogen isotopes. It turned out that the uptake of the amino acid is much more efficient in plants that overproduce the amino acid transporter, and very low in plants lacking the transporter", he explains.
"We grew the plants in greenhouses in agricultural soil, and could follow the way of the amino acid glutamine from the soil into the plant by labelling the glutamine with carbon and nitrogen isotopes. It turned out that the uptake of the amino acid is much more efficient in plants that overproduce the amino acid transporter, and very low in plants lacking the transporter", he explains.
Additionally, the plants that were lacking the transporter had the lowest carbon/nitrogen ratios, and the plants overproducing the transporters had the highest ratios. Theoretically, the amino acids should result in a higher carbon concentration, which indicates that the plants have been taking up organic nitrogen from the soil continuously. "This study is a milestone in our research. With the use of genetically modified model plants we have been able to show that amino acids in soil are used as nitrogen sources by plants. We also aim to increase the plant capacity to take up nitrogen from the soil, and our results show that an optimization of organic nitrogen uptake is a possible way to achieve this", says Torgny Näsholm.
Thale cress is not an agricultural crop, but a model plant which is often used to predict the effects of genetic modifications in agricultural plant species. As part of the research programme Mistra Biotech, the research group now investigates how the uptake of organic nitrogen can be improved in potato. "We are now testing if it is possible to use the same strategy to increase the uptake of organic nitrogen in an agricultural crop. We have propagated modified potato clones to see if we get the same increase in potato as in thale cress."
Photos: Iftikhar Ahmad; Illustration: Ulrika Ganeteg
Scientific article:
Ganeteg, U., Ahmad, I., Jämtgård, S., Aguetoni-Cambui, C., Inselsbacher, E., Svennerstam, H., Schmidt, S., and Näsholm, T. (2017) Amino acid transporter mutants of Arabidopsis provides evidence that a non-mycorrhizal plant acquires organic nitrogen from agricultural soil. Plant, Cell & Environment 40: 413–423. doi: 10.1111/pce.12881.
Link to the Swedish press release on the SLU homepage
More information about Mistra Biotech: http://www.slu.se/mistrabiotech
Photos: Iftikhar Ahmad; Illustration: Ulrika GanetegScientific article:
Ganeteg, U., Ahmad, I., Jämtgård, S., Aguetoni-Cambui, C., Inselsbacher, E., Svennerstam, H., Schmidt, S., and Näsholm, T. (2017) Amino acid transporter mutants of Arabidopsis provides evidence that a non-mycorrhizal plant acquires organic nitrogen from agricultural soil. Plant, Cell & Environment 40: 413–423. doi: 10.1111/pce.12881.
Link to the Swedish press release on the SLU homepage
More information about Mistra Biotech: http://www.slu.se/mistrabiotech
Contacts:
Torgny Näsholm, Professor
Department of Forest Ecology and Management, SLU
Phone: +46-90-786 8205
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Department of Forest Ecology and Management, SLU
Phone: +46-90-786 8205
E-mail:
Ulrika Ganeteg, researcher
Department of Forest Genetics and Plant Physiology, SLU
Phone: +46 (0)90 786 8431
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Department of Forest Genetics and Plant Physiology, SLU
Phone: +46 (0)90 786 8431
E-mail:
Henrik Svennerstam, researcher
Department of Forest Genetics and Plant Physiology, SLU
Phone: +46 (0)90 786 8641
E-mail:This email address is being protected from spambots. You need JavaScript enabled to view it.
Mistra Biotech
This email address is being protected from spambots. You need JavaScript enabled to view it.
+4618 672232
Department of Forest Genetics and Plant Physiology, SLU
Phone: +46 (0)90 786 8641
E-mail:
Mistra Biotech
+4618 672232