{tab=Research} Olivier Keech sitting at the desk in his officePhoto: Fredrik Larsson

Our research explores several aspects of the regulation of plant metabolism in response to stress, with a particular emphasis on mitochondrial metabolism. In plants, the process of aging as well as many environmental constraints may lead to the death of leaves. This particular type of cell death is often referred to as leaf senescence and can have a profoundly negative impact on crop yields and post-harvest shelf-life.

Aim: Leaves are essential plant structures and their well-being is crucial for plant development and survival. When a stress is applied, a plant has two options: try to cope with it or induce senescence and reallocate valuable nutrients towards new, developing or storage organs. A mutual antagonistic relationship can summarize this phenomenon as shown in figure 1. Our aim is to understand how the plant validates senescence over an adaptation strategy in response to stress (Fig. 1). This work mainly covers two aspects: 1) to unveil the communication and signalling mechanisms controlling the induction of leaf senescence and 2) to determine the subsequent metabolic regulation that occurs in response to stress, and ultimately during leaf senescence.

Illustration about the relationship between adaptation and senscence including metabolic/redox balance, hormonal homeostasis, gene regulatory network and different types of stressFigure 1: Mutual antagonistic relationship between adaptation and induction of senescence in response to a stress (e.g. nutrient deficiency, light regime, temperatures, pathogene infection, etc).

1. Using dark-induced senescence as a proxy to decipher signalling pathways controlling the induction of leaf senescence

In earlier studies (Keech et al., 2007; Law et al., 2018), we have shown that a leaf from a plant entirely darkened (DP) can survive much longer than an individually-darkened leaf (IDL; Fig. 2), which suggests that upon the right signals, the induction of leaf senescence can be repressed and alternative metabolic strategies conferring extended longevity can occur.

On the left side, an individual leaf was darkened and this leaf turned yellow after 6 days of treatment. On the right side, an entire plant was darkended and stayed mostly green after 6 days of treatment. Figure 2: Experimental setup for the two darkening treatments (Weaver and Amasino, 2001; Keech et al., 2007; Law et al., 2018).

Yet, our current knowledge on the respective metabolic adjustments remains highly fragmented. In 2018, we proposed the following working models (Fig. 3).

Illustration summarising metabolic strategies in plant leaves to darkeningComic strip by Neil E. Robbins II illustrating the effects of shading in plants

Figure 3: A) Model summarising the different metabolic strategies employed by plants in response to partial or total darkening of the plant. Size and line-weight of the fonts and arrows are proportional to their implication to these metabolic processes. The large arrow behind the leaf in DP conditions depicts the conserved metabolic strategy main-tained between 3 and 6 days of darkening. Abbreviations: AAA - aromatic amino acids, BCAA - branched chain amino acids, Citr - citrate, mETC - mitochondrial electron trans-port chain, OAA - oxaloacetate, PPP - pentose phosphate pathway, Shik/Chor - shikimate/chorismate, TCA - tricarboxylic acid cycle (Law et al., 2018); B) "Are plants afraid of the dark?" Comic strip by Neil E. Robbins II explaining the content of the publication in a humoristic way. Find the full comic strip here: https://neilercomics.com/2018/05/18/are-plants-afraid-of-the-dark/

However, in order to challenge these hypotheses, we are currently investigating the metabolic regulations in a set of functional stay-green mutants issued from a genetic screen. This provides us with a much valuable tool to determine how cells can survive prolonged stress conditions.

2. Regulation of metabolism during leaf senescence

In a green leaf, the three energy organelles (peroxisome, mitochondrion and chloroplast) work in synergy to sustain an efficient assimilation of carbon while constantly maintaining the essential functions of the cell. However, when a leaf undergoes senescence (“yellowing”), whole cell-metabolism is drastically modified, and as chloroplasts are rapidly getting impaired, the remaining organelles acquire novel functions, particularly the mitochondrion. In animals, mitochondria have been shown to integrate various signals and to subsequently modulate cell death processes whereas in plants, the contribution of mitochondria in cell death regulation remains unclear, particularly during leaf senescence.

Therefore, we are currently investigating in more detail the role of mitochondria during both developmental (i.e. aging) and stress-induced leaf senescence (Fig. 4).Illustration of metabolic processes in the mitochondriumFigure 4: Production of glutamate, reducing equivalents and TCA cycle intermediates from catabolic reactions occurring in the mitochondrion during developmental leaf senescence (Chrobok et al., 2016). Transcriptomic overview of the mitochondrially localised portion of the following metabolic pathways: (I) Lysine degradation, (II) branched chain amino acid degradation, (III) D-2HG metabolism, (IV) Glycine and Alanine metabolism, (V) Urea Cycle and (VI) Proline metabolism. Specific genes of these pathways and their transcript abundance during developmental leaf senescence are illustrated here. Production of reducing equivalents is shown as an arrow with an electron (e-).

3. Towards sustainable food production

Among a few other things, we are also interested in complementary alternatives for food production systems. In particular, we are involved in several projects aiming at developing integrated aqua-agro systems in closed land-based units. The strategic implementation of numerous trophic layers within a production system is a natural way to achieve a higher sustainability while maintaining the whole production economically viable.

A concept scheme (Fig. 5), released for the PLATSEN* event end of 2016 depicts some of the interrelationships between the different trophic layers that can be implemented to for example urban farming system in order to achieve a circularity, i.e. a better use of biowaste, energy and resources.

Depiction of the nutrient cycle between the different components of the eMTE modelSchematic overview of the eMulti-Trophic Ecosystem (eMTE) concept

More information about the eMTE project and the exhibition at PLATSEN in 2016.

PLATSEN is thought as a platform where decision makers, politicians, scientists, NGOs and people from public and private sectors can meet and exchange and discuss ideas about sustainability in an urban environment. The 2016 event was initiated by the Swedish Scientific Council for Sustainability in collaboration with several other actors from the public and private sectors e.g. Umeå Municipality and Umeå University.

Integrated fish and plant production workshop 2021

"Towards sustainable urban food production with multi-trophic systems", talk starts at 59 min: Link to the recorded workshop on SLU Play

{tab=Team}
  • Personnel Image
    Boussardon, Clement
    Staff scientist
    E-mail
    Room: B4-34-45
  • Personnel Image
    Cseh, Barnabás
    PhD Student, Representative
    E-mail
    Room: B4-20-45
  • Personnel Image
    Hussain, Shah
    PostDoc
    E-mail
    Room: C4-29-40
  • Personnel Image
    Keech, Olivier
    Associate Professor
    E-mail
    Room: B4-50-45
    Website

{tab=Resources}xlsxList_of_genes_encoding_for_mitochondrial_products_v1.0.xlsx177.41 KB
xlsxSupplemental_Table_1_Law_et_al_2020_DOI_10.3389-fpls_2020:00524.xlsx237.93 MB
xlsxGene_Atlas_of_Fe-containing_proteins_in_Arabidopsis_V1.0.xlsx197.14 KB {tab=Publications}
  2024 (1)
PLANT UNCOUPLING MITOCHONDRIAL PROTEIN 2 localizes to the Golgi. Fuchs, P., Feixes-Prats, E., Arruda, P., Feitosa-Araújo, E., Fernie, A. R, Grefen, C., Lichtenauer, S., Linka, N., de Godoy Maia, I., Meyer, A. J, Schilasky, S., Sweetlove, L. J, Wege, S., Weber, A. P M, Millar, A H., Keech, O., Florez-Sarasa, I., Barreto, P., & Schwarzländer, M. Plant Physiology, 194(2): 623–628. February 2024.
PLANT UNCOUPLING MITOCHONDRIAL PROTEIN 2 localizes to the Golgi [link]Paper   doi   link   bibtex   abstract  
  2023 (3)
Comparison of plastid proteomes points towards a higher plastidial redox turnover in vascular tissues than in mesophyll cells. Boussardon, C., Carrie, C., & Keech, O. Journal of Experimental Botany, 74(14): 4110–4124. August 2023.
Comparison of plastid proteomes points towards a higher plastidial redox turnover in vascular tissues than in mesophyll cells [link]Paper   doi   link   bibtex   abstract  
Mitochondrial ferredoxin-like is essential for forming complex I-containing supercomplexes in Arabidopsis. Röhricht, H., Przybyla-Toscano, J., Forner, J., Boussardon, C., Keech, O., Rouhier, N., & Meyer, E. H Plant Physiology, 191(4): 2170–2184. April 2023.
Mitochondrial ferredoxin-like is essential for forming complex I-containing supercomplexes in Arabidopsis [link]Paper   doi   link   bibtex   abstract  
Tissue-Specific Isolation of Tagged Arabidopsis Plastids. Boussardon, C., & Keech, O. Current Protocols, 3(2): e673. 2023. _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1002/cpz1.673
Tissue-Specific Isolation of Tagged Arabidopsis Plastids [link]Paper   doi   link   bibtex   abstract  
  2022 (5)
Cell Type–Specific Isolation of Mitochondria in Arabidopsis. Boussardon, C., & Keech, O. In Van Aken, O., & Rasmusson, A. G., editor(s), Plant Mitochondria: Methods and Protocols, of Methods in Molecular Biology, pages 13–23. Springer US, New York, NY, January 2022.
Cell Type–Specific Isolation of Mitochondria in Arabidopsis [link]Paper   link   bibtex   abstract  
Maturation and Assembly of Iron-Sulfur Cluster-Containing Subunits in the Mitochondrial Complex I From Plants. López-López, A., Keech, O., & Rouhier, N. Frontiers in Plant Science, 13. May 2022.
Maturation and Assembly of Iron-Sulfur Cluster-Containing Subunits in the Mitochondrial Complex I From Plants [link]Paper   link   bibtex   abstract  
Metabolic control of arginine and ornithine levels paces the progression of leaf senescence. Liebsch, D., Juvany, M., Li, Z., Wang, H., Ziolkowska, A., Chrobok, D., Boussardon, C., Wen, X., Law, S. R, Janečková, H., Brouwer, B., Lindén, P., Delhomme, N., Stenlund, H., Moritz, T., Gardeström, P., Guo, H., & Keech, O. Plant Physiology, 189(4): 1943–1960. August 2022.
Metabolic control of arginine and ornithine levels paces the progression of leaf senescence [link]Paper   doi   link   bibtex   abstract  
Protein lipoylation in mitochondria requires Fe–S cluster assembly factors NFU4 and NFU5. Przybyla-Toscano, J., Maclean, A. E, Franceschetti, M., Liebsch, D., Vignols, F., Keech, O., Rouhier, N., & Balk, J. Plant Physiology, 188(2): 997–1013. February 2022.
Protein lipoylation in mitochondria requires Fe–S cluster assembly factors NFU4 and NFU5 [link]Paper   doi   link   bibtex   abstract  
The RPN12a proteasome subunit is essential for the multiple hormonal homeostasis controlling the progression of leaf senescence. Boussardon, C., Bag, P., Juvany, M., Šimura, J., Ljung, K., Jansson, S., & Keech, O. Communications Biology, 5(1): 1–14. September 2022.
The RPN12a proteasome subunit is essential for the multiple hormonal homeostasis controlling the progression of leaf senescence [link]Paper   doi   link   bibtex   abstract  
  2021 (2)
Gene atlas of iron‐containing proteins in Arabidopsis thaliana. Przybyla‐Toscano, J., Boussardon, C., Law, S. R., Rouhier, N., & Keech, O. The Plant Journal, 106(1): 258–274. April 2021.
Gene atlas of iron‐containing proteins in Arabidopsis thaliana [link]Paper   doi   link   bibtex   2 downloads  
Iron–sulfur proteins in plant mitochondria: roles and maturation. Przybyla-Toscano, J., Christ, L., Keech, O., & Rouhier, N. Journal of Experimental Botany, 72(6): 2014–2044. March 2021.
Iron–sulfur proteins in plant mitochondria: roles and maturation [link]Paper   doi   link   bibtex   abstract   1 download  
  2020 (4)
Centralization Within Sub-Experiments Enhances the Biological Relevance of Gene Co-expression Networks: A Plant Mitochondrial Case Study. Law, S. R., Kellgren, T. G., Björk, R., Ryden, P., & Keech, O. Frontiers in Plant Science, 11: 524. June 2020.
Centralization Within Sub-Experiments Enhances the Biological Relevance of Gene Co-expression Networks: A Plant Mitochondrial Case Study [link]Paper   doi   link   bibtex  
Siberian larch (Larix sibirica Ledeb.) mitochondrial genome assembled using both short and long nucleotide sequence reads is currently the largest known mitogenome. Putintseva, Y. A., Bondar, E. I., Simonov, E. P., Sharov, V. V., Oreshkova, N. V., Kuzmin, D. A., Konstantinov, Y. M., Shmakov, V. N., Belkov, V. I., Sadovsky, M. G., Keech, O., & Krutovsky, K. V. BMC Genomics, 21(1): 654. December 2020.
Siberian larch (Larix sibirica Ledeb.) mitochondrial genome assembled using both short and long nucleotide sequence reads is currently the largest known mitogenome [link]Paper   doi   link   bibtex   abstract  
The Mitogenome of Norway Spruce and a Reappraisal of Mitochondrial Recombination in Plants. Sullivan, A. R, Eldfjell, Y., Schiffthaler, B., Delhomme, N., Asp, T., Hebelstrup, K. H, Keech, O., Öberg, L., Møller, I. M., Arvestad, L., Street, N. R, & Wang, X. Genome Biology and Evolution, 12(1): 3586–3598. January 2020.
The Mitogenome of Norway Spruce and a Reappraisal of Mitochondrial Recombination in Plants [link]Paper   doi   link   bibtex   abstract  
Tissue‐specific isolation of Arabidopsis/plant mitochondria – IMTACT (isolation of mitochondria tagged in specific cell types). Boussardon, C., Przybyla‐Toscano, J., Carrie, C., & Keech, O. The Plant Journal, 103(1): 459–473. July 2020.
Tissue‐specific isolation of Arabidopsis/plant mitochondria – IMTACT (isolation of mitochondria tagged in specific cell types) [link]Paper   doi   link   bibtex   2 downloads  
  2019 (1)
Functional, Structural and Biochemical Features of Plant Serinyl-Glutathione Transferases. Sylvestre-Gonon, E., Law, S. R., Schwartz, M., Robe, K., Keech, O., Didierjean, C., Dubos, C., Rouhier, N., & Hecker, A. Frontiers in Plant Science, 10: 608. May 2019.
Functional, Structural and Biochemical Features of Plant Serinyl-Glutathione Transferases [link]Paper   doi   link   bibtex  
  2018 (1)
Darkened Leaves Use Different Metabolic Strategies for Senescence and Survival. Law, S. R., Chrobok, D., Juvany, M., Delhomme, N., Lindén, P., Brouwer, B., Ahad, A., Moritz, T., Jansson, S., Gardeström, P., & Keech, O. Plant Physiology, 177(1): 132–150. May 2018.
Darkened Leaves Use Different Metabolic Strategies for Senescence and Survival [link]Paper   doi   link   bibtex   2 downloads  
  2017 (2)
In Vitro Alkylation Methods for Assessing the Protein Redox State. Zannini, F., Couturier, J., Keech, O., & Rouhier, N. In Fernie, A. R., Bauwe, H., & Weber, A. P., editor(s), Photorespiration, volume 1653, pages 51–64. Springer New York, New York, NY, 2017. Series Title: Methods in Molecular Biology
In Vitro Alkylation Methods for Assessing the Protein Redox State [link]Paper   doi   link   bibtex  
The redox control of photorespiration: from biochemical and physiological aspects to biotechnological considerations. Keech, O., Gardeström, P., Kleczkowski, L. A., & Rouhier, N. Plant, Cell & Environment, 40(4): 553–569. April 2017.
The redox control of photorespiration: from biochemical and physiological aspects to biotechnological considerations [link]Paper   doi   link   bibtex  
  2016 (7)
Characterization of a novel β-barrel protein (AtOM47) from the mitochondrial outer membrane of Arabidopsis thaliana. Li, L., Kubiszewski-Jakubiak, S., Radomiljac, J., Wang, Y., Law, S. R., Keech, O., Narsai, R., Berkowitz, O., Duncan, O., Murcha, M. W., & Whelan, J. Journal of Experimental Botany, 67(21): 6061–6075. November 2016.
Characterization of a novel β-barrel protein (AtOM47) from the mitochondrial outer membrane of <i>Arabidopsis thaliana</i> [link]Paper   doi   link   bibtex  
Dark‐induced leaf senescence: new insights into a complex light‐dependent regulatory pathway. Liebsch, D., & Keech, O. New Phytologist, 212(3): 563–570. November 2016.
Dark‐induced leaf senescence: new insights into a complex light‐dependent regulatory pathway [link]Paper   doi   link   bibtex  
Dissecting the Metabolic Role of Mitochondria during Developmental Leaf Senescence. Chrobok, D., Law, S. R., Brouwer, B., Lindén, P., Ziolkowska, A., Liebsch, D., Narsai, R., Szal, B., Moritz, T., Rouhier, N., Whelan, J., Gardeström, P., & Keech, O. Plant Physiology, 172(4): 2132–2153. December 2016.
Dissecting the Metabolic Role of Mitochondria during Developmental Leaf Senescence [link]Paper   doi   link   bibtex  
Manipulating photorespiration to increase plant productivity: recent advances and perspectives for crop improvement. Betti, M., Bauwe, H., Busch, F. A., Fernie, A. R., Keech, O., Levey, M., Ort, D. R., Parry, M. A. J., Sage, R., Timm, S., Walker, B., & Weber, A. P. M. Journal of Experimental Botany, 67(10): 2977–2988. May 2016.
Manipulating photorespiration to increase plant productivity: recent advances and perspectives for crop improvement [link]Paper   doi   link   bibtex  
Mitochondrial uncouplers inhibit clathrin-mediated endocytosis largely through cytoplasmic acidification. Dejonghe, W., Kuenen, S., Mylle, E., Vasileva, M., Keech, O., Viotti, C., Swerts, J., Fendrych, M., Ortiz-Morea, F. A., Mishev, K., Delang, S., Scholl, S., Zarza, X., Heilmann, M., Kourelis, J., Kasprowicz, J., Nguyen, L. S. L., Drozdzecki, A., Van Houtte, I., Szatmári, A., Majda, M., Baisa, G., Bednarek, S. Y., Robert, S., Audenaert, D., Testerink, C., Munnik, T., Van Damme, D., Heilmann, I., Schumacher, K., Winne, J., Friml, J., Verstreken, P., & Russinova, E. Nature Communications, 7(1): 11710. September 2016.
Mitochondrial uncouplers inhibit clathrin-mediated endocytosis largely through cytoplasmic acidification [link]Paper   doi   link   bibtex  
Perspectives for a better understanding of the metabolic integration of photorespiration within a complex plant primary metabolism network. Hodges, M., Dellero, Y., Keech, O., Betti, M., Raghavendra, A. S., Sage, R., Zhu, X., Allen, D. K., & Weber, A. P. Journal of Experimental Botany, 67(10): 3015–3026. May 2016.
Perspectives for a better understanding of the metabolic integration of photorespiration within a complex plant primary metabolism network [link]Paper   doi   link   bibtex  
Reduced mitochondrial malate dehydrogenase activity has a strong effect on photorespiratory metabolism as revealed by $^{\textrm{13}}$ C labelling. Lindén, P., Keech, O., Stenlund, H., Gardeström, P., & Moritz, T. Journal of Experimental Botany, 67(10): 3123–3135. May 2016.
Reduced mitochondrial malate dehydrogenase activity has a strong effect on photorespiratory metabolism as revealed by $^{\textrm{13}}$ C labelling [link]Paper   doi   link   bibtex  
  2014 (2)
In response to partial plant shading, the lack of phytochrome A does not directly induce leaf senescence but alters the fine-tuning of chlorophyll biosynthesis. Brouwer, B., Gardeström, P., & Keech, O. Journal of Experimental Botany, 65(14): 4037–4049. July 2014.
In response to partial plant shading, the lack of phytochrome A does not directly induce leaf senescence but alters the fine-tuning of chlorophyll biosynthesis [link]Paper   doi   link   bibtex  
The still mysterious roles of cysteine-containing glutathione transferases in plants. Lallement, P., Brouwer, B., Keech, O., Hecker, A., & Rouhier, N. Frontiers in Pharmacology, 5. August 2014.
The still mysterious roles of cysteine-containing glutathione transferases in plants [link]Paper   doi   link   bibtex  
  2013 (4)
Engineering photorespiration: current state and future possibilities. Peterhansel, C., Krause, K., Braun, H., Espie, G. S., Fernie, A. R., Hanson, D. T., Keech, O., Maurino, V. G., Mielewczik, M., & Sage, R. F. Plant Biology, 15(4): 754–758. July 2013.
Engineering photorespiration: current state and future possibilities [link]Paper   doi   link   bibtex  
Perspectives on plant photorespiratory metabolism. Fernie, A. R., Bauwe, H., Eisenhut, M., Florian, A., Hanson, D. T., Hagemann, M., Keech, O., Mielewczik, M., Nikoloski, Z., Peterhänsel, C., Roje, S., Sage, R., Timm, S., von Cammerer, S., Weber, A. P. M., & Westhoff, P. Plant Biology, 15(4): 748–753. July 2013.
Perspectives on plant photorespiratory metabolism [link]Paper   doi   link   bibtex  
Requirement for the plastidial oxidative pentose phosphate pathway for nitrate assimilation in Arabidopsis. Bussell, J. D., Keech, O., Fenske, R., & Smith, S. M. The Plant Journal, 75(4): 578–591. 2013.
Requirement for the plastidial oxidative pentose phosphate pathway for nitrate assimilation in Arabidopsis [link]Paper   doi   link   bibtex   abstract  
The Norway spruce genome sequence and conifer genome evolution. Nystedt, B., Street, N. R., Wetterbom, A., Zuccolo, A., Lin, Y., Scofield, D. G., Vezzi, F., Delhomme, N., Giacomello, S., Alexeyenko, A., Vicedomini, R., Sahlin, K., Sherwood, E., Elfstrand, M., Gramzow, L., Holmberg, K., Hällman, J., Keech, O., Klasson, L., Koriabine, M., Kucukoglu, M., Käller, M., Luthman, J., Lysholm, F., Niittylä, T., Olson, Å., Rilakovic, N., Ritland, C., Rosselló, J. A., Sena, J., Svensson, T., Talavera-López, C., Theißen, G., Tuominen, H., Vanneste, K., Wu, Z., Zhang, B., Zerbe, P., Arvestad, L., Bhalerao, R. P., Bohlmann, J., Bousquet, J., Garcia Gil, R., Hvidsten, T. R., de Jong, P., MacKay, J., Morgante, M., Ritland, K., Sundberg, B., Lee Thompson, S., Van de Peer, Y., Andersson, B., Nilsson, O., Ingvarsson, P. K., Lundeberg, J., & Jansson, S. Nature, 497(7451): 579–584. May 2013.
The Norway spruce genome sequence and conifer genome evolution [link]Paper   doi   link   bibtex   1 download  
  2012 (2)
The Genetic Dissection of a Short-Term Response to Low CO2 Supports the Possibility for Peroxide-Mediated Decarboxylation of Photorespiratory Intermediates in the Peroxisome. Keech, O., Zhou, W., Fenske, R., Colas-des-Francs-Small, C., Bussell, J. D., Badger, M. R., & Smith, S. M. Molecular Plant, 5(6): 1413–1416. November 2012.
The Genetic Dissection of a Short-Term Response to Low CO2 Supports the Possibility for Peroxide-Mediated Decarboxylation of Photorespiratory Intermediates in the Peroxisome [link]Paper   doi   link   bibtex   1 download  
The impact of light intensity on shade-induced leaf senescence: Light-dependent induction of leaf senescence. Brouwer, B., Ziolkowska, A., Bagard, M., Keech, O., & Gardeström, P. Plant, Cell & Environment, 35(6): 1084–1098. June 2012.
The impact of light intensity on shade-induced leaf senescence: Light-dependent induction of leaf senescence [link]Paper   doi   link   bibtex  
  2011 (1)
The conserved mobility of mitochondria during leaf senescence reflects differential regulation of the cytoskeletal components in Arabidopsis thaliana. Keech, O. Plant Signaling & Behavior, 6(1): 147–150. January 2011.
The conserved mobility of mitochondria during leaf senescence reflects differential regulation of the cytoskeletal components in Arabidopsis thaliana [link]Paper   doi   link   bibtex   abstract  
  2010 (2)
Arabidopsis has a cytosolic fumarase required for the massive allocation of photosynthate into fumaric acid and for rapid plant growth on high nitrogen. Pracharoenwattana, I., Zhou, W., Keech, O., Francisco, P. B., Udomchalothorn, T., Tschoep, H., Stitt, M., Gibon, Y., & Smith, S. M. The Plant Journal, 62(5): 785–795. 2010.
Arabidopsis has a cytosolic fumarase required for the massive allocation of photosynthate into fumaric acid and for rapid plant growth on high nitrogen [link]Paper   doi   link   bibtex   abstract  
Leaf Senescence Is Accompanied by an Early Disruption of the Microtubule Network in Arabidopsis. Keech, O., Pesquet, E., Gutierrez, L., Ahad, A., Bellini, C., Smith, S. M., & Gardeström, P. Plant Physiology, 154(4): 1710–1720. December 2010.
Leaf Senescence Is Accompanied by an Early Disruption of the Microtubule Network in Arabidopsis [link]Paper   doi   link   bibtex   abstract  
  2009 (1)
Magic‐angle phosphorus NMR of functional mitochondria: in situ monitoring of lipid response under apoptotic‐like stress. Sani, M., Keech, O., Gardeström, P., Dufourc, E. J., & Gröbner, G. The FASEB Journal, 23(9): 2872–2878. September 2009.
Magic‐angle phosphorus NMR of functional mitochondria: <i>in situ</i> monitoring of lipid response under apoptotic‐like stress [link]Paper   doi   link   bibtex  
  2007 (2)
The different fates of mitochondria and chloroplasts during dark-induced senescence in Arabidopsis leaves. Keech, O., Pesquet, E., Ahad, A., Askne, A., Nordvall, D., Vodnala, S. M., Tuominen, H., Hurry, V., Dizengremel, P., & Gardeström, P. Plant, Cell & Environment, 30(12): 1523–1534. December 2007.
The different fates of mitochondria and chloroplasts during dark-induced senescence in Arabidopsis leaves [link]Paper   doi   link   bibtex  
The mitochondrial type II peroxiredoxin from poplar. Gama, F., Keech, O., Eymery, F., Finkemeier, I., Gelhaye, E., Gardeström, P., Dietz, K. J., Rey, P., Jacquot, J., & Rouhier, N. Physiologia Plantarum, 129(1): 196–206. January 2007.
The mitochondrial type II peroxiredoxin from poplar [link]Paper   doi   link   bibtex  
  2005 (3)
Adsorption of allelopathic compounds by wood-derived charcoal: the role of wood porosity. Keech, O., Carcaillet, C., & Nilsson, M. Plant and Soil, 272(1): 291–300. May 2005.
Adsorption of allelopathic compounds by wood-derived charcoal: the role of wood porosity [link]Paper   doi   link   bibtex   abstract  
Identification of Plant Glutaredoxin Targets. Rouhier, N., Villarejo, A., Srivastava, M., Gelhaye, E., Keech, O., Droux, M., Finkemeier, I., Samuelsson, G., Dietz, K. J., Jacquot, J., & Wingsle, G. Antioxidants & Redox Signaling, 7(7-8): 919–929. July 2005. Publisher: Mary Ann Liebert, Inc., publishers
Identification of Plant Glutaredoxin Targets [link]Paper   doi   link   bibtex   abstract  
Preparation of leaf mitochondria from Arabidopsis thaliana. Keech, O., Dizengremel, P., & Gardestrom, P. Physiologia Plantarum, 124(4): 403–409. August 2005. Place: Hoboken Publisher: Wiley WOS:000230573300001
doi   link   bibtex   abstract