{tab=Research} Mellerowicz next to aspen trees in the greenhouse

Wood cells accumulate the major proportion of terrestrial biomass in their thick cell walls, composed of cellulose, matrix polysaccharides and lignin. By interacting with cellulose and lignin, matrix polysaccharides affect mechanical and chemical properties of cell walls in developing and mature wood cells, which determines wood quality traits, such as fiber length, wood and fiber strength and durability, and properties important in industrial wood processing such as pulping and saccharification. Our research is aiming at elucidating these matrix functions in wood cells.

CAZymes involved in wood cell wall formation

Matrix polysaccharides are synthesized and modified by Carbohydrate Active Enzymes (CAZYmes) and by other enzymes acting on non-carbohydrate types of side chains. These enzymes reside either in the Golgi apparatus where they synthesize matrix polymers, or in cell walls where they modify them post-synthetically. We have been studying the function of some of these genes expressed during wood cell wall formation using Populus and Arabidopsis thaliana as model species.

Collage of three graphs showing matrix polysaccharide molecules projected on wood cross section on the left, the classification of CAZymes in the middle and a corresponding heat map on the right. Left: Matrix polysaccharides; middle and right: Almost 2000 CAZyme genes classified in super-families were identified in fully sequenced Populus genome. Over 60% of these genes show specific expression pattern during wood biosynthesis indicating their involvement in different wood cell differentiation stages.

Modification of xylan and other matrix polysaccharides in planta

To investigate the role of matrix polysaccharides in mediating cell wall properties we use both, native plant matrix biosynthetic/modifying genes, and microbial transgenes, to modify the structure of matrix polysaccharides in plant cell walls. The microbial enzymes having defined specificity to matrix components in wood, when targeted to cell walls and to developing woody tissues, are a powerful tool to modify specific chains of the matrix. This lets us reveal some of matrix intricate interactions among different wall components and their overall role during wood cell development.

Microscope image of fiber and vessle elements on the left and red coloured wood cells on the right.Left: Matrix modification affects wood cell expansion and thus regulates the size and shape of wood cells. Fibers and vessel elements have different ways of expansion; fibers elongate by intrusive tip growth but vessel elements do not elongate, but they radially expand by a combination of radial intrusive and symplastic growth. Accordingly, a highly regulated modification of matrix in developing wood cells is required to achieve these different outcomes. We found that pectin and xyloglucan modifications are needed for wood cell expansion. Right: Matrix is modified by cell wall-residing enzymes even in the dead cells. Activity of XET enzyme in wood cell walls visualized by the incorporation of the fluorescent substrate to cell walls of tension wood fibers four years after their biosynthesis.

Genetic improvement of aspen for bioprocessing

Aspen tree that is growing in a field trial. Field testing of trees with modified matrix reveals novel functions of matrix that are important in natural environment.

Some of the studied matrix modifications are beneficial for bioprocessing of plant biomass in biorefinery. We have generated many different types of transgenic lines with altered matrix polysaccharides, which had better wood properties based on greenhouse experiments. These lines are subsequently tested in field trials of short rotation. The field trials typically reveal new phenotypes, and are essential for tree improvement as well as for the better understanding of the implication of matrix modification.

Secondary wall integrity sensing?

Transgenic plants with modified cell wall polymers in secondary walls sometimes show increased growth and increased resistance to biotic stresses. The pathways leading to such outcomes are unknown, and we are testing a hypothesis that there exists a type of signaling of secondary cell wall integrity, which is responsible for the observed phenotypical changes. Interestingly, some effects of matrix deficiency are similar to effects of stem swaying. We are currently investigating of the molecular pathways involved in secondary wall integrity sensing and the sensing of stem strains share some common mechanisms.

Key publications

  • Derba-Maceluch M, Amini F, Donev EN, Pawar PM-A, Michaud L, Johansson U, Albrectsen BR and Mellerowicz EJ* (2020) Cell wall acetylation in hybrid aspen affects field performance, foliar phenolic composition and resistance to biological stress factors in a construct-dependent fashion. Frontiers Plant Sci 11:651. https://doi.org/10.3389/fpls.2020.00651
  • Kushwah S, Banasiak A, Nishikubo N, Derba-Maceluch M, Majda M, Endo S, Kumar V, Gomez L, Gorzsás A, McQueen-Mason S, Braam J, Sundberg B, Mellerowicz EJ* (2020). Arabidopsis XTH4 and XTH9 contribute to wood cell expansion and secondary wall formation. Plant Physiology 182: 1946–1965. https://doi.org/10.1104/pp.19.01529
  • Kumar V, Hainaut M, Delhomme N, Mannapperuma C, Street NR, Henrissat B, Mellerowicz EJ* (2019). Poplar Carbohydrate Active Enzymes - new annotation and functional analyses based on RNA expression data. Plant J, 99: 598-609. https://doi.org/10.1111/tpj.14417
  • Ratke C, Terebieniec BK, Winestrand S, Derba-Maceluch M, Grahn T, Schiffthaler B, Ulvcrona T, Özparpucu M, Rüggeberg M, Lundqvist SO, Street NR, Jönsson LJ, Mellerowicz EJ* (2018). Downregulating aspen xylan biosynthetic GT43 genes in developing wood stimulates growth via reprograming of transcriptome. New Phytologist 219: 230-245. https://doi.org/10.1111/nph.15160
  • Leśniewska J, Öhman D, Krzesłowska M, Kushwah S, Barciszewska-Pacak M, Kleczkowski LA, Sundberg B, Moritz T, Mellerowicz EJ* (2017). Defense responses in aspens with altered PME activity reveal the hormonal inducers of tyloses. Plant Physiology 173:1409–1419. https://doi.org/10.1104/pp.16.01443
  • Pawar P M-A, Derba-Maceluch M, Chong SL, Gandla ML, Bashar SS, Sparrman T, Ahvenainen P, Hedenström M, Özparpucu M, Rüggeberg M, Serimaa R, Lawoko M, Tenkanen M, Jönsson LJ, Mellerowicz EJ* (2017). In muro deacetylation of xylan increases lignin extractability and improves saccharification of aspen wood. Biotechnology for Biofuels 10:98. https://doi.org/10.1186/s13068-017-0782-4
  • Pawar P M-A, Ratke C, Balasubramanian VB, Chong SL, Gandla ML, Adriasola M, Sparrman T, Hedenström M, Szwaj K, Derba-Maceluch M, Ezcurra I, Tenkanen M, Jönsson LJ, Mellerowicz EJ* (2017). Downregulation of RWA genes in hybrid aspen affects xylan acetylation and wood processing properties. New Phytologist 214: 1491–1505. https://doi.org/10.1111/nph.14489
{tab=Team}
  • Personnel Image
    Barbut, Félix Robert
    PostDoc
    E-mail
    Room: B5-18-45
  • Personnel Image
    Mellerowicz, Ewa
    Professor
    E-mail
    Room: B6-42-45
    Website
  • Personnel Image
    Mitra, Madhusree
    PostDoc
    E-mail
    Room: B6-16-45
  • Personnel Image
    Pandey, Garima
    PostDoc
    E-mail
    Room: B5-44-45

{tab=CV E. Mellerowicz}
  • 2009: Professor, Swedish University of Agricultural Sciences
  • 2004: Docent, Swedish University of Agricultural Sciences
  • 1999: Assistant Professor, Swedish University of Agricultural Sciences
  • 1998: Research scientist, Swedish University of Agricultural Sciences
  • 1995-97: Research scientist, Forest Research Institute, New Zealand
  • 1992: PhD, University of New Brunswick, Canada 1994: Postdoc, Swedish University of Agricultural Sciences
  • 1986: MSc in Biology, University of New Brunswick, Canada
  • 1979: MSc in Forestry, SGGW, Poland
{tab=Publications}
  2024 (3)
Integrity of xylan backbone affects plant responses to drought. Barbut, F. R., Cavel, E., Donev, E. N., Gaboreanu, I., Urbancsok, J., Pandey, G., Demailly, H., Jiao, D., Yassin, Z., Derba-Maceluch, M., Master, E. R., Scheepers, G., Gutierrez, L., & Mellerowicz, E. J. Frontiers in Plant Science, 15. June 2024. Publisher: Frontiers
Integrity of xylan backbone affects plant responses to drought [link]Paper   doi   link   bibtex   abstract  
Modifying lignin composition and xylan O-acetylation induces changes in cell wall composition, extractability, and digestibility. Chaudhari, A. A., Sharma, A. M., Rastogi, L., Dewangan, B. P., Sharma, R., Singh, D., Sah, R. K., Das, S., Bhattacharjee, S., Mellerowicz, E. J., & Pawar, P. A. Biotechnology for Biofuels and Bioproducts, 17(1): 73. May 2024.
Modifying lignin composition and xylan O-acetylation induces changes in cell wall composition, extractability, and digestibility [link]Paper   doi   link   bibtex   abstract  
Unraveling the unique structural motifs of glucuronoxylan from hybrid aspen wood. Sivan, P., Heinonen, E., Escudero, L., Gandla, M. L., Jiménez-Quero, A., Jönsson, L. J., Mellerowicz, E. J., & Vilaplana, F. Carbohydrate Polymers, 343: 122434. November 2024.
Unraveling the unique structural motifs of glucuronoxylan from hybrid aspen wood [link]Paper   doi   link   bibtex  
  2023 (6)
Biotechnological Potential of the Stress Response and Plant Cell Death Regulators Proteins in the Biofuel Industry. Bernacki, M. J., Mielecki, J., Antczak, A., Drożdżek, M., Witoń, D., Dąbrowska-Bronk, J., Gawroński, P., Burdiak, P., Marchwicka, M., Rusaczonek, A., Dąbkowska-Susfał, K., Strobel, W. R., Mellerowicz, E. J., Zawadzki, J., Szechyńska-Hebda, M., & Karpiński, S. Cells, 12(16): 2018. August 2023. Number: 16 Publisher: Multidisciplinary Digital Publishing Institute
Biotechnological Potential of the Stress Response and Plant Cell Death Regulators Proteins in the Biofuel Industry [link]Paper   doi   link   bibtex   abstract  
Field testing of transgenic aspen from large greenhouse screening identifies unexpected winners. Donev, E. N., Derba-Maceluch, M., Yassin, Z., Gandla, M. L., Pramod, S., Heinonen, E., Kumar, V., Scheepers, G., Vilaplana, F., Johansson, U., Hertzberg, M., Sundberg, B., Winestrand, S., Hörnberg, A., Alriksson, B., Jönsson, L. J., & Mellerowicz, E. J. Plant Biotechnology Journal, 21(5): 1005–1021. 2023. _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/pbi.14012
Field testing of transgenic aspen from large greenhouse screening identifies unexpected winners [link]Paper   doi   link   bibtex   abstract  
Flexure wood formation via growth reprogramming in hybrid aspen involves jasmonates and polyamines and transcriptional changes resembling tension wood development. Urbancsok, J., Donev, E. N., Sivan, P., van Zalen, E., Barbut, F. R., Derba-Maceluch, M., Šimura, J., Yassin, Z., Gandla, M. L., Karady, M., Ljung, K., Winestrand, S., Jönsson, L. J., Scheepers, G., Delhomme, N., Street, N. R., & Mellerowicz, E. J. New Phytologist. October 2023. _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/nph.19307
Flexure wood formation via growth reprogramming in hybrid aspen involves jasmonates and polyamines and transcriptional changes resembling tension wood development [link]Paper   doi   link   bibtex   abstract  
Impact of xylan on field productivity and wood saccharification properties in aspen. Derba-Maceluch, M., Sivan, P., Donev, E. N., Gandla, M. L., Yassin, Z., Vaasan, R., Heinonen, E., Andersson, S., Amini, F., Scheepers, G., Johansson, U., Vilaplana, F. J., Albrectsen, B. R., Hertzberg, M., Jönsson, L. J., & Mellerowicz, E. J. Frontiers in Plant Science, 14. 2023.
Impact of xylan on field productivity and wood saccharification properties in aspen [link]Paper   doi   link   bibtex   abstract  
Sequential extraction of hemicelluloses by subcritical water improves saccharification of hybrid aspen wood grown in greenhouse and field conditions. Sivan, P., Heinonen, E., Gandla, M. L., Jiménez-Quero, A., Özeren, H. D., Jönsson, L. J., Mellerowicz, E. J., & Vilaplana, F. Green Chemistry, 25(14): 5634–5646. July 2023. Publisher: The Royal Society of Chemistry
Sequential extraction of hemicelluloses by subcritical water improves saccharification of hybrid aspen wood grown in greenhouse and field conditions [link]Paper   doi   link   bibtex   abstract  
Xylan glucuronic acid side chains fix suberin-like aliphatic compounds to wood cell walls. Derba-Maceluch, M., Mitra, M., Hedenström, M., Liu, X., Gandla, M. L., Barbut, F. R., Abreu, I. N., Donev, E. N., Urbancsok, J., Moritz, T., Jönsson, L. J., Tsang, A., Powlowski, J., Master, E. R., & Mellerowicz, E. J. New Phytologist, 238(1): 297–312. January 2023. _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/nph.18712
Xylan glucuronic acid side chains fix suberin-like aliphatic compounds to wood cell walls [link]Paper   doi   link   bibtex   abstract  
  2021 (3)
Elongation of wood fibers combines features of diffuse and tip growth. Majda, M., Kozlova, L., Banasiak, A., Derba‐Maceluch, M., Iashchishyn, I. A., Morozova‐Roche, L. A., Smith, R. S., Gorshkova, T., & Mellerowicz, E. J. New Phytologist,nph.17468. May 2021.
Elongation of wood fibers combines features of diffuse and tip growth [link]Paper   doi   link   bibtex   11 downloads  
Saccharification Potential of Transgenic Greenhouse- and Field-Grown Aspen Engineered for Reduced Xylan Acetylation. Pramod, S., Gandla, M. L., Derba-Maceluch, M., Jönsson, L. J., Mellerowicz, E. J., & Winestrand, S. Frontiers in Plant Science, 12: 1722. 2021.
Saccharification Potential of Transgenic Greenhouse- and Field-Grown Aspen Engineered for Reduced Xylan Acetylation [link]Paper   doi   link   bibtex   abstract   3 downloads  
Sucrose synthase determines carbon allocation in developing wood and alters carbon flow at the whole tree level in aspen. Dominguez, P. G., Donev, E., Derba‐Maceluch, M., Bünder, A., Hedenström, M., Tomášková, I., Mellerowicz, E. J., & Niittylä, T. New Phytologist, 229(1): 186–198. January 2021.
Sucrose synthase determines carbon allocation in developing wood and alters carbon flow at the whole tree level in aspen [link]Paper   doi   link   bibtex   8 downloads  
  2020 (6)
Arabidopsis XTH4 and XTH9 Contribute to Wood Cell Expansion and Secondary Wall Formation. Kushwah, S., Banasiak, A., Nishikubo, N., Derba-Maceluch, M., Majda, M., Endo, S., Kumar, V., Gomez, L., Gorzsas, A., McQueen-Mason, S., Braam, J., Sundberg, B., & Mellerowicz, E. J. Plant Physiology, 182(4): 1946–1965. April 2020.
Arabidopsis <i>XTH4</i> and <i>XTH9</i> Contribute to Wood Cell Expansion and Secondary Wall Formation [link]Paper   doi   link   bibtex   5 downloads  
Cell Wall Acetylation in Hybrid Aspen Affects Field Performance, Foliar Phenolic Composition and Resistance to Biological Stress Factors in a Construct-Dependent Fashion. Derba-Maceluch, M., Amini, F., Donev, E. N., Pawar, P. M., Michaud, L., Johansson, U., Albrectsen, B. R., & Mellerowicz, E. J. Frontiers in Plant Science, 11: 651. May 2020.
Cell Wall Acetylation in Hybrid Aspen Affects Field Performance, Foliar Phenolic Composition and Resistance to Biological Stress Factors in a Construct-Dependent Fashion [link]Paper   doi   link   bibtex   4 downloads  
Expression of Cell Wall–Modifying Enzymes in Aspen for Improved Lignocellulose Processing. Derba-Maceluch, M., & Mellerowicz, E. J. In Popper, Z. A., editor(s), The Plant Cell Wall, volume 2149, pages 145–164. Springer New York, New York, NY, 2020. Series Title: Methods in Molecular Biology
Expression of Cell Wall–Modifying Enzymes in Aspen for Improved Lignocellulose Processing [link]Paper   doi   link   bibtex   3 downloads  
Genetic control of tracheid properties in Norway spruce wood. Baison, J., Zhou, L., Forsberg, N., Mörling, T., Grahn, T., Olsson, L., Karlsson, B., Wu, H. X., Mellerowicz, E. J., Lundqvist, S., & García-Gil, M. R. Scientific Reports, 10(1): 18089. December 2020.
Genetic control of tracheid properties in Norway spruce wood [link]Paper   doi   link   bibtex   abstract   1 download  
Genome-Wide Identification of Populus Malectin/Malectin-Like Domain-Containing Proteins and Expression Analyses Reveal Novel Candidates for Signaling and Regulation of Wood Development. Kumar, V., Donev, E. N., Barbut, F. R., Kushwah, S., Mannapperuma, C., Urbancsok, J., & Mellerowicz, E. J. Frontiers in Plant Science, 11: 588846. December 2020.
Genome-Wide Identification of Populus Malectin/Malectin-Like Domain-Containing Proteins and Expression Analyses Reveal Novel Candidates for Signaling and Regulation of Wood Development [link]Paper   doi   link   bibtex   abstract  
Hybrid Aspen Expressing a Carbohydrate Esterase Family 5 Acetyl Xylan Esterase Under Control of a Wood-Specific Promoter Shows Improved Saccharification. Wang, Z., Pawar, P. M., Derba-Maceluch, M., Hedenström, M., Chong, S., Tenkanen, M., Jönsson, L. J., & Mellerowicz, E. J. Frontiers in Plant Science, 11: 380. April 2020.
Hybrid Aspen Expressing a Carbohydrate Esterase Family 5 Acetyl Xylan Esterase Under Control of a Wood-Specific Promoter Shows Improved Saccharification [link]Paper   doi   link   bibtex   3 downloads  
  2019 (1)
Poplar carbohydrate‐active enzymes: whole‐genome annotation and functional analyses based on RNA expression data. Kumar, V., Hainaut, M., Delhomme, N., Mannapperuma, C., Immerzeel, P., Street, N. R., Henrissat, B., & Mellerowicz, E. J. The Plant Journal, 99(4): 589–609. August 2019.
Poplar carbohydrate‐active enzymes: whole‐genome annotation and functional analyses based on RNA expression data [link]Paper   doi   link   bibtex  
  2018 (7)
Downregulating aspen xylan biosynthetic GT43 genes in developing wood stimulates growth via reprograming of the transcriptome. Ratke, C., Terebieniec, B. K., Winestrand, S., Derba-Maceluch, M., Grahn, T., Schiffthaler, B., Ulvcrona, T., Özparpucu, M., Rüggeberg, M., Lundqvist, S., Street, N. R., Jönsson, L. J., & Mellerowicz, E. J. New Phytologist, 219(1): 230–245. July 2018.
Downregulating aspen xylan biosynthetic GT43 genes in developing wood stimulates growth via reprograming of the transcriptome [link]Paper   doi   link   bibtex   1 download  
Engineering Non-cellulosic Polysaccharides of Wood for the Biorefinery. Donev, E., Gandla, M. L., Jönsson, L. J., & Mellerowicz, E. J. Frontiers in Plant Science, 9: 1537. October 2018.
Engineering Non-cellulosic Polysaccharides of Wood for the Biorefinery [link]Paper   doi   link   bibtex  
Ethylene signaling induces gelatinous layers with typical features of tension wood in hybrid aspen. Felten, J., Vahala, J., Love, J., Gorzsás, A., Rüggeberg, M., Delhomme, N., Leśniewska, J., Kangasjärvi, J., Hvidsten, T. R., Mellerowicz, E. J., & Sundberg, B. New Phytologist, 218(3): 999–1014. May 2018.
Ethylene signaling induces gelatinous layers with typical features of tension wood in hybrid aspen [link]Paper   doi   link   bibtex   2 downloads  
Genetic control of transition from juvenile to mature wood with respect to microfibril angle in Norway spruce ( Picea abies ) and lodgepole pine ( Pinus contorta ). Hayatgheibi, H., Forsberg, N. E. G., Lundqvist, S., Mörling, T., Mellerowicz, E. J., Karlsson, B., Wu, H. X., & García-Gil, M. R. Canadian Journal of Forest Research, 48(11): 1358–1365. November 2018.
Genetic control of transition from juvenile to mature wood with respect to microfibril angle in Norway spruce ( <i>Picea abies</i> ) and lodgepole pine ( <i>Pinus contorta</i> ) [link]Paper   doi   link   bibtex   abstract  
PtxtPME1 and homogalacturonans influence xylem hydraulic properties in poplar. Allario, T., Tixier, A., Awad, H., Lemaire, C., Brunel, N., Badel, E., Barigah, T. S., Julien, J., Peyret, P., Mellerowicz, E. J., Cochard, H., & Herbette, S. Physiologia Plantarum, 163(4): 502–515. 2018. _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/ppl.12702
PtxtPME1 and homogalacturonans influence xylem hydraulic properties in poplar [link]Paper   doi   link   bibtex   abstract  
QTL Mapping of Wood FT-IR Chemotypes Shows Promise for Improving Biofuel Potential in Short Rotation Coppice Willow (Salix spp.). Pawar, P. M., Schnürer, A., Mellerowicz, E. J., & Rönnberg-Wästljung, A. C. BioEnergy Research, 11(2): 351–363. June 2018.
QTL Mapping of Wood FT-IR Chemotypes Shows Promise for Improving Biofuel Potential in Short Rotation Coppice Willow (Salix spp.) [link]Paper   doi   link   bibtex   abstract  
Transcriptional induction of cell wall remodelling genes is coupled to microtubule-driven growth isotropy at the shoot apex in Arabidopsis. Armezzani, A., Abad, U., Ali, O., Robin, A. A., Vachez, L., Larrieu, A., Mellerowicz, E. J., Taconnat, L., Battu, V., Stanislas, T., Liu, M., Vernoux, T., Traas, J., & Sassi, M. Development,dev.162255. January 2018.
Transcriptional induction of cell wall remodelling genes is coupled to microtubule-driven growth isotropy at the shoot apex in Arabidopsis [link]Paper   doi   link   bibtex   abstract  
  2017 (7)
A collection of genetically engineered Populus trees reveals wood biomass traits that predict glucose yield from enzymatic hydrolysis. Escamez, S., Latha Gandla, M., Derba-Maceluch, M., Lundqvist, S., Mellerowicz, E. J., Jönsson, L. J., & Tuominen, H. Scientific Reports, 7(1): 15798. December 2017.
A collection of genetically engineered Populus trees reveals wood biomass traits that predict glucose yield from enzymatic hydrolysis [link]Paper   doi   link   bibtex   1 download  
AspWood: High-Spatial-Resolution Transcriptome Profiles Reveal Uncharacterized Modularity of Wood Formation in Populus tremula. Sundell, D., Street, N. R., Kumar, M., Mellerowicz, E. J., Kucukoglu, M., Johnsson, C., Kumar, V., Mannapperuma, C., Delhomme, N., Nilsson, O., Tuominen, H., Pesquet, E., Fischer, U., Niittylä, T., Sundberg, B., & Hvidsten, T. R. The Plant Cell, 29(7): 1585–1604. July 2017.
AspWood: High-Spatial-Resolution Transcriptome Profiles Reveal Uncharacterized Modularity of Wood Formation in Populus tremula [link]Paper   doi   link   bibtex  
Defense Responses in Aspen with Altered Pectin Methylesterase Activity Reveal the Hormonal Inducers of Tyloses. Leśniewska, J., Öhman, D., Krzesłowska, M., Kushwah, S., Barciszewska-Pacak, M., Kleczkowski, L. A., Sundberg, B., Moritz, T., & Mellerowicz, E. J. Plant Physiology, 173(2): 1409–1419. February 2017.
Defense Responses in Aspen with Altered Pectin Methylesterase Activity Reveal the Hormonal Inducers of Tyloses [link]Paper   doi   link   bibtex   1 download  
Downregulation of RWA genes in hybrid aspen affects xylan acetylation and wood saccharification. Pawar, P. M., Ratke, C., Balasubramanian, V. K., Chong, S., Gandla, M. L., Adriasola, M., Sparrman, T., Hedenström, M., Szwaj, K., Derba-Maceluch, M., Gaertner, C., Mouille, G., Ezcurra, I., Tenkanen, M., Jönsson, L. J., & Mellerowicz, E. J. New Phytologist, 214(4): 1491–1505. 2017. _eprint: https://nph.onlinelibrary.wiley.com/doi/pdf/10.1111/nph.14489
Downregulation of RWA genes in hybrid aspen affects xylan acetylation and wood saccharification [link]Paper   doi   link   bibtex   abstract  
Downregulation of \textlessspan style="font-variant:small-caps;"\textgreaterRWA\textless/span\textgreater genes in hybrid aspen affects xylan acetylation and wood saccharification. Pawar, P. M., Ratke, C., Balasubramanian, V. K., Chong, S., Gandla, M. L., Adriasola, M., Sparrman, T., Hedenström, M., Szwaj, K., Derba‐Maceluch, M., Gaertner, C., Mouille, G., Ezcurra, I., Tenkanen, M., Jönsson, L. J., & Mellerowicz, E. J. New Phytologist, 214(4): 1491–1505. June 2017.
Downregulation of \textlessspan style="font-variant:small-caps;"\textgreaterRWA\textless/span\textgreater genes in hybrid aspen affects xylan acetylation and wood saccharification [link]Paper   doi   link   bibtex  
In muro deacetylation of xylan affects lignin properties and improves saccharification of aspen wood. Pawar, P. M., Derba-Maceluch, M., Chong, S., Gandla, M. L., Bashar, S. S., Sparrman, T., Ahvenainen, P., Hedenström, M., Özparpucu, M., Rüggeberg, M., Serimaa, R., Lawoko, M., Tenkanen, M., Jönsson, L. J., & Mellerowicz, E. J. Biotechnology for Biofuels, 10(1): 98. December 2017.
In muro deacetylation of xylan affects lignin properties and improves saccharification of aspen wood [link]Paper   doi   link   bibtex  
Mechanochemical Polarization of Contiguous Cell Walls Shapes Plant Pavement Cells. Majda, M., Grones, P., Sintorn, I., Vain, T., Milani, P., Krupinski, P., Zagórska-Marek, B., Viotti, C., Jönsson, H., Mellerowicz, E. J., Hamant, O., & Robert, S. Developmental Cell, 43(3): 290–304.e4. November 2017.
Mechanochemical Polarization of Contiguous Cell Walls Shapes Plant Pavement Cells [link]Paper   doi   link   bibtex  
  2016 (5)
An efficient method for medium throughput screening of cuticular wax composition in different plant species. Fernandez-Moreno, J., Malitsky, S., Lashbrooke, J., Biswal, A. K., Racovita, R. C., Mellerowicz, E. J., Jetter, R., Orzaez, D., Aharoni, A., & Granell, A. Metabolomics, 12(4): 73. April 2016.
An efficient method for medium throughput screening of cuticular wax composition in different plant species [link]Paper   doi   link   bibtex  
Expression of fungal acetyl xylan esterase in Arabidopsis thaliana improves saccharification of stem lignocellulose. Pawar, P. M., Derba-Maceluch, M., Chong, S., Gómez, L. D., Miedes, E., Banasiak, A., Ratke, C., Gaertner, C., Mouille, G., McQueen-Mason, S. J., Molina, A., Sellstedt, A., Tenkanen, M., & Mellerowicz, E. J. Plant Biotechnology Journal, 14(1): 387–397. January 2016.
Expression of fungal acetyl xylan esterase in <i>Arabidopsis thaliana</i> improves saccharification of stem lignocellulose [link]Paper   doi   link   bibtex  
Genetic analysis of fiber dimensions and their correlation with stem diameter and solid-wood properties in Norway spruce. Chen, Z., Karlsson, B., Mörling, T., Olsson, L., Mellerowicz, E. J., Wu, H. X., Lundqvist, S., & Gil, M. R. G. Tree Genetics & Genomes, 12(6): 123. December 2016.
Genetic analysis of fiber dimensions and their correlation with stem diameter and solid-wood properties in Norway spruce [link]Paper   doi   link   bibtex  
Method for accurate fiber length determination from increment cores for large-scale population analyses in Norway spruce. Chen, Z., Abramowicz, K., Raczkowski, R., Ganea, S., Wu, H. X., Lundqvist, S., Mörling, T., de Luna, S. S., García Gil, M. R., & Mellerowicz, E. J. Holzforschung, 70(9): 829–838. September 2016.
Method for accurate fiber length determination from increment cores for large-scale population analyses in Norway spruce [link]Paper   doi   link   bibtex   abstract  
Pectinous cell wall thickenings formation – A common defense strategy of plants to cope with Pb. Krzesłowska, M., Rabęda, I., Basińska, A., Lewandowski, M., Mellerowicz, E. J., Napieralska, A., Samardakiewicz, S., & Woźny, A. Environmental Pollution, 214: 354–361. July 2016.
Pectinous cell wall thickenings formation – A common defense strategy of plants to cope with Pb [link]Paper   doi   link   bibtex  
  2015 (7)
Active fungal GH115 alpha-glucuronidase produced in Arabidopsis thaliana affects only the UX1-reactive glucuronate decorations on native glucuronoxylans. Chong, S. L., Derba-Maceluch, M., Koutaniemi, S., Gomez, L. D., McQueen-Mason, S. J., Tenkanen, M., & Mellerowicz, E. J. BMC Biotechnol, 15(1): 56. June 2015. Edition: 2015/06/19
Active fungal GH115 alpha-glucuronidase produced in Arabidopsis thaliana affects only the UX1-reactive glucuronate decorations on native glucuronoxylans [link]Paper   doi   link   bibtex   abstract  
Aspen Tension Wood Fibers Contain beta-(1—\textgreater 4)-Galactans and Acidic Arabinogalactans Retained by Cellulose Microfibrils in Gelatinous Walls. Gorshkova, T., Mokshina, N., Chernova, T., Ibragimova, N., Salnikov, V., Mikshina, P., Tryfona, T., Banasiak, A., Immerzeel, P., Dupree, P., & Mellerowicz, E. J. Plant Physiol, 169(3): 2048–63. November 2015. Edition: 2015/09/18
Aspen Tension Wood Fibers Contain beta-(1—\textgreater 4)-Galactans and Acidic Arabinogalactans Retained by Cellulose Microfibrils in Gelatinous Walls [link]Paper   doi   link   bibtex   abstract  
Colocalization of low-methylesterified pectins and Pb deposits in the apoplast of aspen roots exposed to lead. Rabeda, I., Bilski, H., Mellerowicz, E. J., Napieralska, A., Suski, S., Wozny, A., & Krzeslowska, M. Environ Pollut, 205: 315–26. October 2015. Edition: 2015/07/01
Colocalization of low-methylesterified pectins and Pb deposits in the apoplast of aspen roots exposed to lead [link]Paper   doi   link   bibtex   abstract   1 download  
Expression of a fungal glucuronoyl esterase in Populus: effects on wood properties and saccharification efficiency. Latha Gandla, M., Derba-Maceluch, M., Liu, X., Gerber, L., Master, E. R., Mellerowicz, E. J., & Jonsson, L. J. Phytochemistry, 112: 210–20. April 2015. Edition: 2014/07/07
Expression of a fungal glucuronoyl esterase in Populus: effects on wood properties and saccharification efficiency [link]Paper   doi   link   bibtex   abstract   1 download  
Glucuronic acid in Arabidopsis thaliana xylans carries a novel pentose substituent. Chong, S. L., Koutaniemi, S., Juvonen, M., Derba-Maceluch, M., Mellerowicz, E. J., & Tenkanen, M. Int J Biol Macromol, 79: 807–12. August 2015. Edition: 2015/06/07
Glucuronic acid in Arabidopsis thaliana xylans carries a novel pentose substituent [link]Paper   doi   link   bibtex   abstract  
Populus GT43 family members group into distinct sets required for primary and secondary wall xylan biosynthesis and include useful promoters for wood modification. Ratke, C., Pawar, P. M., Balasubramanian, V. K., Naumann, M., Duncranz, M. L., Derba-Maceluch, M., Gorzsas, A., Endo, S., Ezcurra, I., & Mellerowicz, E. J. Plant Biotechnol J, 13(1): 26–37. January 2015. Edition: 2014/08/08
Populus GT43 family members group into distinct sets required for primary and secondary wall xylan biosynthesis and include useful promoters for wood modification [link]Paper   doi   link   bibtex   abstract  
Suppression of xylan endotransglycosylase PtxtXyn10A affects cellulose microfibril angle in secondary wall in aspen wood. Derba‐Maceluch, M., Awano, T., Takahashi, J., Lucenius, J., Ratke, C., Kontro, I., Busse‐Wicher, M., Kosik, O., Tanaka, R., Winzéll, A., Kallas, Å., Leśniewska, J., Berthold, F., Immerzeel, P., Teeri, T. T., Ezcurra, I., Dupree, P., Serimaa, R., & Mellerowicz, E. J. New Phytologist, 205(2): 666–681. January 2015.
Suppression of xylan endotransglycosylase <i>PtxtXyn10A</i> affects cellulose microfibril angle in secondary wall in aspen wood [link]Paper   doi   link   bibtex   1 download  
  2013 (4)
Acetylation of woody lignocellulose: significance and regulation. Pawar, P. M., Koutaniemi, S., Tenkanen, M., & Mellerowicz, E. J. Frontiers in Plant Science, 4. 2013.
Acetylation of woody lignocellulose: significance and regulation [link]Paper   doi   link   bibtex   1 download  
Aspen SUCROSE TRANSPORTER3 Allocates Carbon into Wood Fibers. Mahboubi, A., Ratke, C., Gorzsas, A., Kumar, M., Mellerowicz, E. J., & Niittylä, T. PLANT PHYSIOLOGY, 163(4): 1729–1740. December 2013.
Aspen SUCROSE TRANSPORTER3 Allocates Carbon into Wood Fibers [link]Paper   doi   link   bibtex  
Reduced Wall Acetylation Proteins Play Vital and Distinct Roles in Cell Wall O -Acetylation in Arabidopsis. Manabe, Y., Verhertbruggen, Y., Gille, S., Harholt, J., Chong, S., Pawar, P. M., Mellerowicz, E. J., Tenkanen, M., Cheng, K., Pauly, M., & Scheller, H. V. Plant Physiology, 163(3): 1107–1117. October 2013.
Reduced Wall Acetylation Proteins Play Vital and Distinct Roles in Cell Wall <i>O</i> -Acetylation in Arabidopsis [link]Paper   doi   link   bibtex   abstract  
Xyloglucan endotransglucosylase/hydrolase (XTH) overexpression affects growth and cell wall mechanics in etiolated Arabidopsis hypocotyls. Miedes, E., Suslov, D., Vandenbussche, F., Kenobi, K., Ivakov, A., Van Der Straeten, D., Lorences, E. P., Mellerowicz, E. J., Verbelen, J., & Vissenberg, K. Journal of Experimental Botany, 64(8): 2481–2497. May 2013.
Xyloglucan endotransglucosylase/hydrolase (XTH) overexpression affects growth and cell wall mechanics in etiolated Arabidopsis hypocotyls [link]Paper   doi   link   bibtex   1 download  
  2012 (3)
Constitutive expression of a fungal glucuronoyl esterase in Arabidopsis reveals altered cell wall composition and structure: Transgenic expression of PcGCE in Arabidopsis. Tsai, A. Y., Canam, T., Gorzsás, A., Mellerowicz, E. J., Campbell, M. M., & Master, E. R. Plant Biotechnology Journal, 10(9): 1077–1087. December 2012.
Constitutive expression of a fungal glucuronoyl esterase in Arabidopsis reveals altered cell wall composition and structure: Transgenic expression of PcGCE in Arabidopsis [link]Paper   doi   link   bibtex  
Plant Fiber Formation: State of the Art, Recent and Expected Progress, and Open Questions. Gorshkova, T., Brutch, N., Chabbert, B., Deyholos, M., Hayashi, T., Lev-Yadun, S., Mellerowicz, E. J., Morvan, C., Neutelings, G., & Pilate, G. Critical Reviews in Plant Sciences, 31(3): 201–228. May 2012.
Plant Fiber Formation: State of the Art, Recent and Expected Progress, and Open Questions [link]Paper   doi   link   bibtex  
Tensional stress generation in gelatinous fibres: a review and possible mechanism based on cell-wall structure and composition. Mellerowicz, E. J., & Gorshkova, T. A. Journal of Experimental Botany, 63(2): 551–565. January 2012.
Tensional stress generation in gelatinous fibres: a review and possible mechanism based on cell-wall structure and composition [link]Paper   doi   link   bibtex   3 downloads  
  2011 (4)
Pectin Methylesterase Is Induced in Arabidopsis upon Infection and Is Necessary for a Successful Colonization by Necrotrophic Pathogens. Raiola, A., Lionetti, V., Elmaghraby, I., Immerzeel, P., Mellerowicz, E. J., Salvi, G., Cervone, F., & Bellincampi, D. Molecular Plant-Microbe Interactions®, 24(4): 432–440. April 2011.
Pectin Methylesterase Is Induced in <i>Arabidopsis</i> upon Infection and Is Necessary for a Successful Colonization by Necrotrophic Pathogens [link]Paper   doi   link   bibtex   abstract  
Reduced Expression of the SHORT-ROOT Gene Increases the Rates of Growth and Development in Hybrid Poplar and Arabidopsis. Wang, J., Andersson-Gunnerås, S., Gaboreanu, I., Hertzberg, M., Tucker, M. R., Zheng, B., Leśniewska, J., Mellerowicz, E. J., Laux, T., Sandberg, G., & Jones, B. PLoS ONE, 6(12): e28878. December 2011.
Reduced Expression of the SHORT-ROOT Gene Increases the Rates of Growth and Development in Hybrid Poplar and Arabidopsis [link]Paper   doi   link   bibtex  
Xyloglucan endo -Transglycosylase-Mediated Xyloglucan Rearrangements in Developing Wood of Hybrid Aspen. Nishikubo, N., Takahashi, J., Roos, A. A., Derba-Maceluch, M., Piens, K., Brumer, H., Teeri, T. T., Stålbrand, H., & Mellerowicz, E. J. Plant Physiology, 155(1): 399–413. January 2011.
Xyloglucan <i>endo</i> -Transglycosylase-Mediated Xyloglucan Rearrangements in Developing Wood of Hybrid Aspen [link]Paper   doi   link   bibtex   abstract  
Xyloglucan endo-Transglycosylase-Mediated Xyloglucan Rearrangements in Developing Wood of Hybrid Aspen. Nishikubo, N., Takahashi, J., Roos, A. A., Derba-Maceluch, M., Piens, K., Brumer, H., Teeri, T. T., Stålbrand, H., & Mellerowicz, E. J. Plant Physiology, 155(1): 399–413. January 2011.
Xyloglucan endo-Transglycosylase-Mediated Xyloglucan Rearrangements in Developing Wood of Hybrid Aspen [link]Paper   doi   link   bibtex   abstract  
  2009 (3)
A Real-Time Fluorogenic Assay for the Visualization of Glycoside Hydrolase Activity in Planta. Ibatullin, F. M., Banasiak, A., Baumann, M. J., Greffe, L., Takahashi, J., Mellerowicz, E. J., & Brumer, H. Plant Physiology, 151(4): 1741–1750. December 2009.
A Real-Time Fluorogenic Assay for the Visualization of Glycoside Hydrolase Activity in Planta [link]Paper   doi   link   bibtex  
KORRIGAN1 and its Aspen Homolog PttCel9A1 Decrease Cellulose Crystallinity in Arabidopsis Stems. Takahashi, J., Rudsander, U. J., Hedenström, M., Banasiak, A., Harholt, J., Amelot, N., Immerzeel, P., Ryden, P., Endo, S., Ibatullin, F. M., Brumer, H., del Campillo, E., Master, E. R., Vibe Scheller, H., Sundberg, B., Teeri, T. T., & Mellerowicz, E. J. Plant and Cell Physiology, 50(6): 1099–1115. June 2009.
KORRIGAN1 and its Aspen Homolog PttCel9A1 Decrease Cellulose Crystallinity in Arabidopsis Stems [link]Paper   doi   link   bibtex   1 download  
UDP-Glucose Pyrophosphorylase is not Rate Limiting, but is Essential in Arabidopsis. Meng, M., Geisler, M., Johansson, H., Harholt, J., Scheller, H. V., Mellerowicz, E. J., & Kleczkowski, L. A. Plant and Cell Physiology, 50(5): 998–1011. May 2009.
UDP-Glucose Pyrophosphorylase is not Rate Limiting, but is Essential in Arabidopsis [link]Paper   doi   link   bibtex  
  2008 (4)
Microgenomic analysis reveals cell type‐specific gene expression patterns between ray and fusiform initials within the cambial meristem of Populus. Goué, N., Lesage‐Descauses, M., Mellerowicz, E. J., Magel, E., Label, P., & Sundberg, B. New Phytologist, 180(1): 45–56. October 2008.
Microgenomic analysis reveals cell type‐specific gene expression patterns between ray and fusiform initials within the cambial meristem of <i>Populus</i> [link]Paper   doi   link   bibtex  
Pectin Methyl Esterase Inhibits Intrusive and Symplastic Cell Growth in Developing Wood Cells of Populus. Siedlecka, A., Wiklund, S., Péronne, M., Micheli, F., Leśniewska, J., Sethson, I., Edlund, U., Richard, L., Sundberg, B., & Mellerowicz, E. J. Plant Physiology, 146(2): 323–324. February 2008.
Pectin Methyl Esterase Inhibits Intrusive and Symplastic Cell Growth in Developing Wood Cells of <i>Populus</i> [link]Paper   doi   link   bibtex   abstract   4 downloads  
Visualization of GC/TOF-MS-Based Metabolomics Data for Identification of Biochemically Interesting Compounds Using OPLS Class Models. Wiklund, S., Johansson, E., Sjöström, L., Mellerowicz, E. J., Edlund, U., Shockcor, J. P., Gottfries, J., Moritz, T., & Trygg, J. Analytical Chemistry, 80(1): 115–122. January 2008.
Visualization of GC/TOF-MS-Based Metabolomics Data for Identification of Biochemically Interesting Compounds Using OPLS Class Models [link]Paper   doi   link   bibtex  
Xyloglucan: The Molecular Muscle of Trees. Mellerowicz, E. J., Immerzeel, P., & Hayashi, T. Annals of Botany, 102(5): 659–665. November 2008.
Xyloglucan: The Molecular Muscle of Trees [link]Paper   doi   link   bibtex  
  2007 (3)
Differential tissue/organ-dependent expression of two sucrose- and cold-responsive genes for UDP-glucose pyrophosphorylase in Populus. Meng, M., Geisler, M., Johansson, H., Mellerowicz, E. J., Karpinski, S., & Kleczkowski, L. A. Gene, 389(2): 186–195. March 2007.
Differential tissue/organ-dependent expression of two sucrose- and cold-responsive genes for UDP-glucose pyrophosphorylase in Populus [link]Paper   doi   link   bibtex  
Ectopic expression of a wood-abundant expansin PttEXPA1 promotes cell expansion in primary and secondary tissues in aspen. Gray-Mitsumune, M., Blomquist, K., McQueen-Mason, S., Teeri, T. T., Sundberg, B., & Mellerowicz, E. J. Plant Biotechnology Journal, 0(0): 071003005211001–???. October 2007.
Ectopic expression of a wood-abundant expansin PttEXPA1 promotes cell expansion in primary and secondary tissues in aspen [link]Paper   doi   link   bibtex  
Xyloglucan Endo-transglycosylase (XET) Functions in Gelatinous Layers of Tension Wood Fibers in Poplar—A Glimpse into the Mechanism of the Balancing Act of Trees. Nishikubo, N., Awano, T., Banasiak, A., Bourquin, V., Ibatullin, F., Funada, R., Brumer, H., Teeri, T. T., Hayashi, T., Sundberg, B., & Mellerowicz, E. J. Plant and Cell Physiology, 48(6): 843–855. June 2007.
Xyloglucan Endo-transglycosylase (XET) Functions in Gelatinous Layers of Tension Wood Fibers in Poplar—A Glimpse into the Mechanism of the Balancing Act of Trees [link]Paper   doi   link   bibtex  
  2006 (2)
Biosynthesis of cellulose-enriched tension wood in Populus: global analysis of transcripts and metabolites identifies biochemical and developmental regulators in secondary wall biosynthesis. Andersson-Gunnerås, S., Mellerowicz, E. J., Love, J., Segerman, B., Ohmiya, Y., Coutinho, P. M., Nilsson, P., Henrissat, B., Moritz, T., & Sundberg, B. The Plant Journal, 45(2): 144–165. 2006. _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-313X.2005.02584.x
Biosynthesis of cellulose-enriched tension wood in Populus: global analysis of transcripts and metabolites identifies biochemical and developmental regulators in secondary wall biosynthesis [link]Paper   doi   link   bibtex   abstract  
Poplar carbohydrate-active enzymes. Gene identification and expression analyses. Geisler-Lee, J., Geisler, M., Coutinho, P. M., Segerman, B., Nishikubo, N., Takahashi, J., Aspeborg, H., Djerbi, S., Master, E., Andersson-Gunneras, S., Sundberg, B., Karpinski, S., Teeri, T. T., Kleczkowski, L. A., Henrissat, B., & Mellerowicz, E. J. Plant Physiology, 140(3): 946–962. March 2006. Place: Rockville Publisher: Amer Soc Plant Biologists WOS:000235868900014
doi   link   bibtex   abstract  
  2004 (2)
Expansins Abundant in Secondary Xylem Belong to Subgroup A of the α-Expansin Gene Family. Gray-Mitsumune, M., Mellerowicz, E. J., Abe, H., Schrader, J., Winzéll, A., Sterky, F., Blomqvist, K., McQueen-Mason, S., Teeri, T. T., & Sundberg, B. Plant Physiology, 135(3): 1552–1564. July 2004.
Expansins Abundant in Secondary Xylem Belong to Subgroup A of the α-Expansin Gene Family [link]Paper   doi   link   bibtex   abstract  
Liquid Phase Fluorescence in situ RT-PCR Analysis for Gene Expression Analysis in Woody Stems. Gray-Mitsumune, M., Abe, H., Takahashi, J., Sundberg, B., & Mellerowicz, E. J. Plant Biology, 6(1): 47–54. 2004. _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1055/s-2003-44747
Liquid Phase Fluorescence in situ RT-PCR Analysis for Gene Expression Analysis in Woody Stems [link]Paper   doi   link   bibtex   abstract  
  2002 (1)
Xyloglucan Endotransglycosylases Have a Function during the Formation of Secondary Cell Walls of Vascular Tissues. Bourquin, V., Nishikubo, N., Abe, H., Brumer, H., Denman, S., Eklund, M., Christiernin, M., Teeri, T. T., Sundberg, B., & Mellerowicz, E. J. The Plant Cell, 14(12): 3073–3088. December 2002.
Xyloglucan Endotransglycosylases Have a Function during the Formation of Secondary Cell Walls of Vascular Tissues [link]Paper   doi   link   bibtex   abstract  
  2001 (1)
Unravelling cell wall formation in the woody dicot stem. Mellerowicz, E. J., Baucher, M., Sundberg, B., & Boerjan, W. Plant Molecular Biology, 47(1-2): 239–274. September 2001.
link   bibtex   abstract  
  1998 (1)
PRFLL– a Pinus radiata homologue of FLORICAULA and LEAFY is expressed in buds containing vegetative shoot and undifferentiated male cone primordia. Mellerowicz, E. J., Horgan, K., Walden, A., Coker, A., & Walter, C. Planta, 206(4): 619–629. October 1998.
PRFLL– a Pinus radiata homologue of FLORICAULA and LEAFY is expressed in buds containing vegetative shoot and undifferentiated male cone primordia [link]Paper   doi   link   bibtex   abstract  
  1996 (1)
Changes in nuclear genome size and relative ribosomal RNA gene content in cambial region cells of Abies balsamea shoots during the development of dormancy. Lloyd, A. D., Little, C. H. A., Mellerowicz, E. J., & Riding, R. T. Canadian Journal of Botany, 74(2): 290–298. February 1996. Publisher: NRC Research Press
Changes in nuclear genome size and relative ribosomal RNA gene content in cambial region cells of Abies balsamea shoots during the development of dormancy [link]Paper   doi   link   bibtex   abstract  
  1995 (2)
Nuclear and cytoplasmic changes associated with maturation in the vascular cambium of Larix laricina. Mellerowicz, E. J., Riding, R. T., & Greenwood, M. S. Tree Physiology, 15(7-8): 443–449. July 1995.
Nuclear and cytoplasmic changes associated with maturation in the vascular cambium of Larix laricina [link]Paper   doi   link   bibtex   abstract  
Seasonal variation in the nuclear genome size of ray cells in the vascular cambium of Fraxinus americana. Zhong, Y., Mellerowicz, E. J., Lloyd, A. D., Leinhos, V., Riding, R. T., & Little, C. H. A. Physiologia Plantarum, 93(2): 305–311. 1995. _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1399-3054.1995.tb02233.x
Seasonal variation in the nuclear genome size of ray cells in the vascular cambium of Fraxinus americana [link]Paper   doi   link   bibtex   abstract  
  1994 (1)
Fluctuations in ribosomal RNA gene content and nucleolar activity in the cambial region of Abies balsamea (Pinaceae) shoots during reactivation. Lloyd, A. D., Mellerowicz, E. J., Chow, C. H., Riding, R. T., & Little, C. H. A. American Journal of Botany, 81(11): 1384–1389. 1994. _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1002/j.1537-2197.1994.tb15622.x
Fluctuations in ribosomal RNA gene content and nucleolar activity in the cambial region of Abies balsamea (Pinaceae) shoots during reactivation [link]Paper   doi   link   bibtex   abstract  
  1993 (1)
Nucleolar activity in the fusiform cambial cells of Abies balsamea (Pinaceae): effect of season and age. Mellerowicz, E. J., Riding, R. T., & Little, C. H. A. American Journal of Botany, 80(10): 1168–1174. 1993. _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1002/j.1537-2197.1993.tb15349.x
Nucleolar activity in the fusiform cambial cells of Abies balsamea (Pinaceae): effect of season and age [link]Paper   doi   link   bibtex   abstract  
  1992 (3)
Does DNA Endoreduplication Occur During Differentiation of Secondary Xylem and Phloem in Abies balsamea?. Mellerowicz, E. J., & Riding, R. T. International Journal of Plant Sciences, 153(1): 26–30. March 1992. Publisher: The University of Chicago Press
Does DNA Endoreduplication Occur During Differentiation of Secondary Xylem and Phloem in Abies balsamea? [link]Paper   doi   link   bibtex   abstract  
Periodicity of cambial activity in Abies balsamea. I. Effects of temperature and photoperiod on cambial dormancy and frost hardiness. Mellerowicz, E. J., Coleman, W. K., Riding, R. T., & Little, C. H. A. Physiologia Plantarum, 85(3): 515–525. 1992. _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1399-3054.1992.tb05820.x
Periodicity of cambial activity in Abies balsamea. I. Effects of temperature and photoperiod on cambial dormancy and frost hardiness [link]Paper   doi   link   bibtex   abstract  
Periodicity of cambial activity in Abies balsamea. II. Effects of temperature and photoperiod on the size of the nuclear genome in fusiform cambial cells. Mellerowicz, E. J., Riding, R. T., & Little, C. H. A. Physiologia Plantarum, 85(3): 526–530. 1992. _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1399-3054.1992.tb05821.x
Periodicity of cambial activity in Abies balsamea. II. Effects of temperature and photoperiod on the size of the nuclear genome in fusiform cambial cells [link]Paper   doi   link   bibtex   abstract  
  1990 (1)
Nuclear size and shape changes in fusiform cambial cells of Abies balsamea during the annual cycle of activity and dormancy. Mellerowicz, E. J., Riding, R. T., & Little, C. H. A. Canadian Journal of Botany, 68(9): 1857–1863. September 1990. Publisher: NRC Research Press
Nuclear size and shape changes in fusiform cambial cells of Abies balsamea during the annual cycle of activity and dormancy [link]Paper   doi   link   bibtex   abstract  
  1989 (1)
Genomic variability in the vascular cambium of Abies balsamea. Mellerowicz, E. J., Riding, R. T., & Little, C. H. A. Canadian Journal of Botany, 67(4): 990–996. April 1989. Publisher: NRC Research Press
Genomic variability in the vascular cambium of Abies balsamea [link]Paper   doi   link   bibtex   abstract  
  undefined (1)
Modification of xylan in secondary walls alters cell wall biosynthesis and wood formation programs and improves saccharification. Sivan, P., Urbancsok, J., Donev, E. N., Derba-Maceluch, M., Barbut, F. R., Yassin, Z., Gandla, M. L., Mitra, M., Heinonen, S. E., Šimura, J., Cermanová, K., Karady, M., Scheepers, G., Jönsson, L. J., Master, E. R., Vilaplana, F., & Mellerowicz, E. J. Plant Biotechnology Journal, n/a(n/a). . _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1111/pbi.14487
Modification of xylan in secondary walls alters cell wall biosynthesis and wood formation programs and improves saccharification [link]Paper   doi   link   bibtex   abstract  
{tab=Svenska} Ewa Mellerowicz bredvid aspträd i växthus

Vedråvara är en viktig förnyelsebar råvara och innehåller organiskt kol i form av cellulosa, lignin och hemicellulosor. Xylan är den vanligaste hemicellulosan i veden och har stor potential som grön energi och råvara för nya polymerer. Det är därför av intresse att förstå biosyntesen av xylan.

Xylan är uppbyggt av xylos i form av en polymer med sidokedjor, som är viktiga för xylanets egenskaper. Xylanet polymeriseras inuti cellen och matas ut i cellväggen där det sätts ihop i cellväggens nätverk. Vi avser att modifiera xylanets struktur och sammansättning i asp träd genom att använda de gener som kodar för enzymer i xylanbiosyntesen. Vår målsättning är också att upptäcka nya gener som är viktiga för xylanbiosyntes.

Flera gener som fungerar i xylanbiosyntesen har nyligen upptäckts. Genom att använda bioinformatik kan vi leta efter andra gener som regleras på samma sätt, och därför med viss sannolikhet även de har en funktion i xylanbiosyntesen.