Cutting Edge Seminar - Natasha Raikhel
Date:
Tuesday, October 18, 2011 10:00 - 11:00
Duration:
1 Hour
Categories:
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Contact info
Rishi Bhalerao
Title: Dissecting the Endomembrane Network via Chemical Genomics
Lecturer: Natasha Raikhel, University of California Riverside, USA
Place: KB3A9 "Lilla hörsalen", KBC
Abstract:
Although it is known that proteins are delivered to and recycled from the
plasma membrane (PM) via endosomes, the nature of the compartments and
pathways responsible for cargo and vesicle sorting and cellular signaling is
poorly understood. Such highly dynamic processes are not easily approached
genetically. To define and dissect specific recycling pathways, rapid-acting
chemical affecters of proteins involved in vesicle trafficking, especially
through endosomes, would be invaluable. Thus, we identified chemicals
affecting essential steps in PM/endosome trafficking by utilizing the
intensely localized PM transport at the tips of germinating tobacco pollen
tubes. We screened diverse chemical libraries for those that interfered with
pollen germination and tip growth. We found that many also had effects in
Arabidopsis roots for which there are several well-characterized marker
proteins that cycle to and from the plasma membrane. Recent results from our
screens will be discussed.
Lecturer: Natasha Raikhel, University of California Riverside, USA
Place: KB3A9 "Lilla hörsalen", KBC
Abstract:
Although it is known that proteins are delivered to and recycled from the
plasma membrane (PM) via endosomes, the nature of the compartments and
pathways responsible for cargo and vesicle sorting and cellular signaling is
poorly understood. Such highly dynamic processes are not easily approached
genetically. To define and dissect specific recycling pathways, rapid-acting
chemical affecters of proteins involved in vesicle trafficking, especially
through endosomes, would be invaluable. Thus, we identified chemicals
affecting essential steps in PM/endosome trafficking by utilizing the
intensely localized PM transport at the tips of germinating tobacco pollen
tubes. We screened diverse chemical libraries for those that interfered with
pollen germination and tip growth. We found that many also had effects in
Arabidopsis roots for which there are several well-characterized marker
proteins that cycle to and from the plasma membrane. Recent results from our
screens will be discussed.