Microscopy Facility

All users are reminded to acknowledge in publications, presentations and posters on the role of the facilities and expertise. 
"The authors acknowledge the facilities and technical assistance of the Umeå Plant Science Centre (UPSC) Microscopy Facility”

The microscopy facilities at UPSC include a broad range of equipment for sample preparation, subsequent analysis and image acquisition. We work with different sample sizes, starting from thick sections (hundret of μm) semi thin sections (20 to 0.5 um) and even with ultrathin sections or nanoresolution (AFM). Users of the microscopy facilities can perform their experiments after appropriate introduction to the different instruments. We are here to introduce you to fascinating micrsocopy woarld, to advise, to give a proper training according to your needs and to advice during experiental procedures.

Whole cross section of poplar stem from microscope, stained with safranin and alcianblue.Cross section of poplar stem, stained with safranin and alcianblue.Whole cross section of poplar stem from microscope, stained for starch.Cross section of poplar stem, stained for starch.

Available equipment:

1. Microscopes

  1. Three Zeiss Axioplan (fluorescence) microscopes with digital camera systems

  2. Stereomicroscopes

  3. Epifluorescence microscope Leica DMi8 fully motorized with two digital cameras

  4. Fluorescence stereomicroscope Leica M205 FA fully motorized with two digital cameras  and Leica AFL III

2. Confocal laser scanning microscopes

  1. (CSLM)A. Zeiss LSM780 CLSM with inverted stand

  2. Zeiss LSM880 CLSM with airyscan, airyfast, FLIM, FCS, FCCS and inverted stand

  3. Zeiss LSM800 CLSM with airyscan and upright stand

  4. Leica Stellaris 8 DIVE 

  5. Nikon AZ-Z2 vertical macroconfocal

  6.  Macroconfocal Leica LSI HSC

  7. Image analysis computer with full version of Zeiss and Leica softwares

3. Atomic Force Microscope

  1. NanoWizard® 4 XP BioScience, BioScience with VortisTM2 Advanced​ version SPM Controller, HybridStageTM, DirectOverlayTM 2 software module and QITM- Advanced 2 software module

4. Sectioning devices

  1. Two Leica vibratomes VT1000S

  2. Power Tom XL ultramicrotome for resin embedded material

  3. Zeiss HM 350 microtome for wax embedded material sectioning

  4. Cryostat - Cryo Star NX70 with CroJane tap system

  5. Glass Knife Maker

5. Automated in situ hybridization or immuno-histochemistry labelling

  1. Intavis InsituPro VSi automated system

6. Fluorescence Activated Cell Sorter (FACS)

  1. BD FACSAria Flow Cytometer

For more information of the equipment see respective description in the menu to the right. For introductions or questions please contact concerned contact person.


we2smallPersonel working at the facility, from left: Marta Derba-Maceluch, Stephanie Robert and Anna Gustavsson.











Contact:
Stephanie Robert, Professor
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Anna Gustavsson, 1st Research Engineer, 25% (CLSM)
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Marta Derba-Maceluch, scientist (AFM, light microscopy and sectioning)
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Ioanna Antoniadi, (FACS)
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Siamsa Doyle, postdoc (InsituPro VSi)
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